pubmed: lupus and stem cell ...
NCBI: db=pubmed; Term=lupus and stem cell therapy
NCBI pubmed
  • In-depth characterization of CD24(high)CD38(high) transitional human B cells reveals different regulatory profiles.
    Related Articles

    In-depth characterization of CD24(high)CD38(high) transitional human B cells reveals different regulatory profiles.

    J Allergy Clin Immunol. 2016 May;137(5):1577-1584.e10

    Authors: Simon Q, Pers JO, Cornec D, Le Pottier L, Mageed RA, Hillion S

    Abstract
    BACKGROUND: CD24(high)CD38(high) transitional B cells represent cells at a key stage in their developmental pathway. In addition, these B cells have been widely ascribed regulatory functions and involvement in the control of chronic inflammatory diseases. However, the phenotypic and functional overlap between these cells and regulatory B cells remains controversial.
    OBJECTIVE: In this study we wanted to explore the regulatory properties of CD24(high)CD38(high) human B cells.
    METHODS: We used multicolor flow cytometry in combination with bioinformatics and functional studies to show that CD24(high)CD38(high) B cells can be distinguished into multiple subsets with different regulatory functions.
    RESULTS: For the first time, the study reveals that human transitional B cells encompass not only transitional type 1 and type 2 B cells, as previously suggested, but also distinct anergic type 3 B cells, as well as IL-10-producing CD27(+) transitional B cells. Interestingly, the latter 2 subsets differentially regulate CD4(+) T-cell proliferation and polarization toward TH1 effector cells. Additional analyses reveal that the percentage of type 3 B cells is reduced and the frequency of CD27(+) transitional B cells is increased in patients with autoimmune diseases compared with those in matched healthy subjects.
    CONCLUSION: This study provides evidence for the existence of different transitional B-cell subsets, each displaying unique phenotypic and regulatory functional profiles. Furthermore, the study indicates that altered distribution of transitional B-cell subsets highlights different regulatory defects in patients with different autoimmune diseases.

    PMID: 26525227 [PubMed - indexed for MEDLINE]

pubmed: lupus and stem cell ...
NCBI: db=pubmed; Term=lupus and stem cell treatment
NCBI pubmed
  • Umbilical cord mesenchymal stem cells for the treatment of autoimmune diseases: beware of cell-to-cell contact.
    Related Articles

    Umbilical cord mesenchymal stem cells for the treatment of autoimmune diseases: beware of cell-to-cell contact.

    Ann Rheum Dis. 2017 Jun 13;:

    Authors: Alunno A, Bistoni O, Montanucci P, Basta G, Calafiore R, Gerli R

    PMID: 28611081 [PubMed - as supplied by publisher]

  • In-depth characterization of CD24(high)CD38(high) transitional human B cells reveals different regulatory profiles.
    Related Articles

    In-depth characterization of CD24(high)CD38(high) transitional human B cells reveals different regulatory profiles.

    J Allergy Clin Immunol. 2016 May;137(5):1577-1584.e10

    Authors: Simon Q, Pers JO, Cornec D, Le Pottier L, Mageed RA, Hillion S

    Abstract
    BACKGROUND: CD24(high)CD38(high) transitional B cells represent cells at a key stage in their developmental pathway. In addition, these B cells have been widely ascribed regulatory functions and involvement in the control of chronic inflammatory diseases. However, the phenotypic and functional overlap between these cells and regulatory B cells remains controversial.
    OBJECTIVE: In this study we wanted to explore the regulatory properties of CD24(high)CD38(high) human B cells.
    METHODS: We used multicolor flow cytometry in combination with bioinformatics and functional studies to show that CD24(high)CD38(high) B cells can be distinguished into multiple subsets with different regulatory functions.
    RESULTS: For the first time, the study reveals that human transitional B cells encompass not only transitional type 1 and type 2 B cells, as previously suggested, but also distinct anergic type 3 B cells, as well as IL-10-producing CD27(+) transitional B cells. Interestingly, the latter 2 subsets differentially regulate CD4(+) T-cell proliferation and polarization toward TH1 effector cells. Additional analyses reveal that the percentage of type 3 B cells is reduced and the frequency of CD27(+) transitional B cells is increased in patients with autoimmune diseases compared with those in matched healthy subjects.
    CONCLUSION: This study provides evidence for the existence of different transitional B-cell subsets, each displaying unique phenotypic and regulatory functional profiles. Furthermore, the study indicates that altered distribution of transitional B-cell subsets highlights different regulatory defects in patients with different autoimmune diseases.

    PMID: 26525227 [PubMed - indexed for MEDLINE]