pubmed: meniscus and stem ce...
NCBI: db=pubmed; Term=meniscus and stem cell treatment
NCBI pubmed
  • Do cell based Tissue Engineering products for meniscus regeneration influence vascularization?
    Related Articles

    Do cell based Tissue Engineering products for meniscus regeneration influence vascularization?

    Clin Hemorheol Microcirc. 2017 Jun 07;:

    Authors: Koch M, Ehrenreich T, Koehl G, Pattappa G, Pfeifer C, Loibl M, Müller M, Nerlich M, Angele P, Zellner J

    Abstract
    BACKGROUND: Meniscus regeneration is observed within the peripheral, vascularized zone but decreases in the inner two thirds alongside the vascularization. Within this avascular area, cell-based tissue-engineering-approaches appears to be a promising strategy for the treatment of meniscal defects.
    OBJECTIVE: Evaluation of the angiogenic potential of cell-based tissue-engineering-products for meniscus healing.
    METHODS: Evaluation of angiogenesis induced by rabbit meniscus-pellets, meniscus-cells (MC) or mesenchymal stem-cells (MSC) in cell-based tissue-engineering-products within a rabbit meniscus-ring was performed using a transparent dorsal skin fold chamber in nude mice. Observations were undertaken during a 14 days period. Cell preconditioning differed between experimental groups. Immunohistochemical analysis of the regenerated tissue in the meniscus-ring induced by cell loaded composite scaffolds for differentiation and anti-angiogenic factors were performed.
    RESULTS: Meniscus-pellets and MSC-/MC-based tissue-engineering-products induced angiogenesis. An accelerated vascularization was detected in the group of meniscus-pellets derived from the vascularized zone compared to avascular meniscus-pellets. In terms of cell-based tissue-engineering-products, chondrogenic preconditioning resulted in significantly increased vessel growth. MSC-constructs showed an accelerated angiogenesis. Immunohistochemical evaluation showed a progressive differentiation and lower content for anti-angiogenic endostatin in the precultured group.
    CONCLUSIONS: Preconditioning of MC-/MSC-based tissue-engineering-products is a promising tool to influence the angiogenic potential of tissue-engineering-products and to adapt these properties according to the aimed tissue qualities.

    PMID: 28598834 [PubMed - as supplied by publisher]

pubmed: meniscus and stem ce...
NCBI: db=pubmed; Term=meniscus and stem cell treatment
NCBI pubmed
  • Do cell based Tissue Engineering products for meniscus regeneration influence vascularization?
    Related Articles

    Do cell based Tissue Engineering products for meniscus regeneration influence vascularization?

    Clin Hemorheol Microcirc. 2017 Jun 07;:

    Authors: Koch M, Ehrenreich T, Koehl G, Pattappa G, Pfeifer C, Loibl M, Müller M, Nerlich M, Angele P, Zellner J

    Abstract
    BACKGROUND: Meniscus regeneration is observed within the peripheral, vascularized zone but decreases in the inner two thirds alongside the vascularization. Within this avascular area, cell-based tissue-engineering-approaches appears to be a promising strategy for the treatment of meniscal defects.
    OBJECTIVE: Evaluation of the angiogenic potential of cell-based tissue-engineering-products for meniscus healing.
    METHODS: Evaluation of angiogenesis induced by rabbit meniscus-pellets, meniscus-cells (MC) or mesenchymal stem-cells (MSC) in cell-based tissue-engineering-products within a rabbit meniscus-ring was performed using a transparent dorsal skin fold chamber in nude mice. Observations were undertaken during a 14 days period. Cell preconditioning differed between experimental groups. Immunohistochemical analysis of the regenerated tissue in the meniscus-ring induced by cell loaded composite scaffolds for differentiation and anti-angiogenic factors were performed.
    RESULTS: Meniscus-pellets and MSC-/MC-based tissue-engineering-products induced angiogenesis. An accelerated vascularization was detected in the group of meniscus-pellets derived from the vascularized zone compared to avascular meniscus-pellets. In terms of cell-based tissue-engineering-products, chondrogenic preconditioning resulted in significantly increased vessel growth. MSC-constructs showed an accelerated angiogenesis. Immunohistochemical evaluation showed a progressive differentiation and lower content for anti-angiogenic endostatin in the precultured group.
    CONCLUSIONS: Preconditioning of MC-/MSC-based tissue-engineering-products is a promising tool to influence the angiogenic potential of tissue-engineering-products and to adapt these properties according to the aimed tissue qualities.

    PMID: 28598834 [PubMed - as supplied by publisher]