pubmed: osteoarthritis and s...
NCBI: db=pubmed; Term=osteoarthritis and stem cell therapy
NCBI pubmed
  • Phage Display Technology in Biomaterials Engineering: Progress and Opportunities for Applications in Regenerative Medicine.
    Related Articles

    Phage Display Technology in Biomaterials Engineering: Progress and Opportunities for Applications in Regenerative Medicine.

    ACS Chem Biol. 2016 Nov 18;11(11):2962-2980

    Authors: Martins IM, Reis RL, Azevedo HS

    Abstract
    The field of regenerative medicine has been gaining momentum steadily over the past few years. The emphasis in regenerative medicine is to use various in vitro and in vivo approaches that leverage the intrinsic healing mechanisms of the body to treat patients with disabling injuries and chronic diseases such as diabetes, osteoarthritis, and degenerative disorders of the cardiovascular and central nervous system. Phage display has been successfully employed to identify peptide ligands for a wide variety of targets, ranging from relatively small molecules (enzymes, cell receptors) to inorganic, organic, and biological (tissues) materials. Over the past two decades, phage display technology has advanced tremendously and has become a powerful tool in the most varied fields of research, including biotechnology, materials science, cell biology, pharmacology, and diagnostics. The growing interest in and success of phage display libraries is largely due to its incredible versatility and practical use. This review discusses the potential of phage display technology in biomaterials engineering for applications in regenerative medicine.

    PMID: 27661443 [PubMed - indexed for MEDLINE]

pubmed: osteoarthritis and s...
NCBI: db=pubmed; Term=osteoarthritis and stem cell treatment
NCBI pubmed
  • Anti-inflammatory and anti-osteoarthritis effects of tectorigenin.
    Related Articles

    Anti-inflammatory and anti-osteoarthritis effects of tectorigenin.

    Biol Open. 2017 Jun 22;:

    Authors: Wang CL, Li, Wang CD, Xiao F, Zhu JF, Shen C, Zuo B, Cui YM, Wang H, Gao Y, Hu GL, Zhang XL, Chen XD

    Abstract
    Osteoarthritis (OA) is a common and dynamic joint disease, including the articular cartilage, underlying bones, and synovium. In particular, OA is considered as the degeneration of the cartilage. Tectorigenin (Tec) can affect many biological processes. However, its effect on articular chondrocytes remains unclear. This study aimed to assess the role of Tec in articular cartilage. In vitro, Tec inhibited the expression levels of type X collagen, cyclooxigenase-2, matrix metalloproteinase (MMP)-3, and MMP-13 gene but enhanced those of Runx1, type II collagen, and aggrecan in the presence of IL-1β. Meanwhile, Tec inhibited apoptosis through the Bax/Bcl-2/caspase-3 pathway, upregulating p-Bad, downregulating Bax/Bcl-2 ratio, and activating caspase-3 compared with IL-1β treatment only. Moreover, this process was partially regulated by NF-κB P65. In vivo, the chondroprotective effect of Tec was assessed by establishing a model of surgically induced OA. Tec-treated joints exhibited fewer osteoarthritic changes than saline-treated joints. Meanwhile, 1.5 µg/kg Tec treatment produced better protective effect than 0.75 µg/kg. Osteoarthritis Research Society International scoring system were employed to assess histopathological grading of the models , as well as the outcomes of immunohistochemistry for Aggrecan Neoepitope and MMP-3, further confirmed the results. In conclusion, this study showed that Tec played a chondroprotective role on the OA process by preventing articular cartilage degeneration and chondrocyte apoptosis via the NF-κB P65 pathway.

    PMID: 28642243 [PubMed - as supplied by publisher]

  • Phage Display Technology in Biomaterials Engineering: Progress and Opportunities for Applications in Regenerative Medicine.
    Related Articles

    Phage Display Technology in Biomaterials Engineering: Progress and Opportunities for Applications in Regenerative Medicine.

    ACS Chem Biol. 2016 Nov 18;11(11):2962-2980

    Authors: Martins IM, Reis RL, Azevedo HS

    Abstract
    The field of regenerative medicine has been gaining momentum steadily over the past few years. The emphasis in regenerative medicine is to use various in vitro and in vivo approaches that leverage the intrinsic healing mechanisms of the body to treat patients with disabling injuries and chronic diseases such as diabetes, osteoarthritis, and degenerative disorders of the cardiovascular and central nervous system. Phage display has been successfully employed to identify peptide ligands for a wide variety of targets, ranging from relatively small molecules (enzymes, cell receptors) to inorganic, organic, and biological (tissues) materials. Over the past two decades, phage display technology has advanced tremendously and has become a powerful tool in the most varied fields of research, including biotechnology, materials science, cell biology, pharmacology, and diagnostics. The growing interest in and success of phage display libraries is largely due to its incredible versatility and practical use. This review discusses the potential of phage display technology in biomaterials engineering for applications in regenerative medicine.

    PMID: 27661443 [PubMed - indexed for MEDLINE]